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93968ES60Recombinant Biotinylated Human IGF1R/CD221 Protein
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產(chǎn)品簡介
| 供貨周期 | 現(xiàn)貨 | 貨號 | 93968ES60 |
|---|---|---|---|
| 應(yīng)用領(lǐng)域 | 醫(yī)療衛(wèi)生,生物產(chǎn)業(yè),制藥/生物制藥 |
產(chǎn)品介紹
性能參數(shù)
表達(dá)區(qū)間及表達(dá)系統(tǒng)（Source） | Biotinylated Human IGF1R/CD221 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Glu31-Asn932.[Accession | P08069] |
分子量大小（Molecular Weight） | The protein has a predicted MW of 105.8 kDa (alpha subunit) and 23 kDa (beta subunit). Due to glycosylation, the protein migrates to 110-120 kDa (alpha subunit) and 52-55 kDa (beta subunit) based on Tris-Bis PAGE result. |
內(nèi)毒素（Endotoxin） | <1EU per μg by the LAL method. |
純度（Purity） | > 95% as determined by Tris-Bis PAGE and HPLC |
活性（Activity） | ELISA Data: Immobilized Anti-IGF1R Antibody at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human IGF1R, His Tag with the EC50 of 48.0ng/ml determined by ELISA. ELISA Data:Immobilized Biotinylated Human IGF1R, His Tag at 5μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Human IGF-I, hFc Tag with the EC50 of 0.73μg/ml determined by ELISA. |
制劑（Formulation） | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
重構(gòu)方法（Reconstitution） | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
儲存條件
The product should be stored at -25~-15℃ for 1 year from date of receipt.
2-7 days, 2 ~ 8 °C under sterile conditions after reconstitution.
3 months, -25~-15℃ under sterile conditions after reconstitution.
Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.
注意事項(xiàng)
1.Please operate with lab coats and disposable gloves,for your safety.
2.This product is for research use only.
Ver. CN20230721
