BD Ms F4/80 PE T45-2342 100ug 565410

貨號： 565410
品牌： BD Pharmingen
同種亞型： Rat WI, also known as Wistar (outbred) IgG2a, κ
反應(yīng)種屬： Mouse (QC Testing)
應(yīng)用： Flow cytometry (Routinely Tested)

Two-color flow cytometric analysis of F4/80 expression on mouse splenic leucocytes. C57BL/6 mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody (Mouse BD Fc Block™) (Cat. No. 553141/553142). The cells were then stained with Alexa Fluor® 647 Rat Anti-Mouse CD11b (Cat. No. 557686) and either PE Rat IgG2a Isotype Control (Cat. No. 553930; Left Panel) or PE Rat Anti-Mouse F4/80 antibody (Cat. No. 565410; Right Panel). The two-color flow cytometric contour plot showing the correlated expression of F4/80 (or Ig Isotype control staining) versus CD11b was derived from gated events with the forward and side light-scatter characteristics of viable monocytes. Flow cytometric analysis was performed using a BD LSRFortessa™ Cell Analyzer System.