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BD Pharminge FITC Hamster Anti-Mouse CD3e 561827
熒光素標(biāo)記
FITC
抗原名稱
CD3E
宿主
Armenian Hamster IgG1, κ
免疫原
H-2Kb specific cytotoxic T lymphocyte clone BM10-37
別名
CD3; CD3 epsilon; Cd3e; CD3ε; T3e
BD Pharminge FITC Hamster Anti-Mouse CD3e 561827
抗體詳情:
145-2C11
The 145-2C11 monoclonal antibody specifically binds to the 25-kDa ε chain of the T-cell receptor-associated CD3 complex that is expressed on thymocytes, mature T lymphocytes, and NK-T cells. The cytoplasmic domain of CD3e participates in the signal transduction events that activate several cellular biochemical pathways as a result of antigen recognition. Soluble 145-2C11 antibody can activate either unprimed (naive) or primed (memory/preactivated) T cells in vivo or in vitro, in the presence of Fc receptor-bearing accessory cells. In contrast, plate-bound 145-2C11 can activate T cells in the absence of accessory cells. Soluble 145-2C11 antibody has been reported to induce re-directed lysis of Fc receptor-bearing target cells by CTL clones and can also block lysis of specific target cells by antigen-specific CTL's. Under some conditions, T-cell activation by 145-2C11 antibody has been reported to result in apoptotic cell death. The 145-2C11 antibody does not cross-react with rat leukocytes. Preincubation of thymus cell suspensions at 37°C for 2-4 hours prior to staining reportedly enhances the ability of anti-CD3ε and anti-αβ TCR mAbs to detect the T-cell receptor on immature thymocytes.
Multicolor flow cytometric analysis of CD3e expression on mouse splenocytes. Mouse splenic leucocytes were stained with APC Rat Anti-Mouse CD4 (Cat. No. 553051/561091) and APC Rat Anti-Mouse CD8a (Cat. No. 553035/561093) antibodies and either FITC Armenian Hamster IgG1, κ Isotype Control (Cat. No. 553971; Left Plot) or FITC Hamster Anti-Mouse CD3e antibody (Cat. No. 553062/553061/561827; Right Plot) at 0.5 µg/test. Bivariate pseudocolor density plots showing the correlated expression of CD3e (or Ig Isotype Control staining) versus CD4 and CD8a were derived from gated events with the forward and side light-scatter characteristics of intact splenic leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ software. Data shown on this Technical Data Sheet are not lot specific.